Objectives: To compare the in vitro antibacterial efficacy of antistaphylococcal antibiotics in combination with
fosfomycin or rifampicin, using a biofilm model.
Methods: The antibacterial activities of fusidic acid, linezolid, vancomycin, teicoplanin, rifampicin, minocycline,
fosfomycin and tigecycline, individually and in fosfomycin or rifampicin combinations, were measured against
planktonic or biofilm-embedded methicillin-resistant Staphylococcus aureus (MRSA) with susceptible and resist-
ant breakpoint concentrations (SBCs and RBCs, respectively), using the MTT-staining method and by counting
the number of cfu in the biofilms.
Results:LinezolidaloneatitsSBC,andfosfomycin,linezolid, minocyclineandtigecyclineattheirRBCs,exhibited
killing effects on biofilm-embedded MRSA (P,0.0001). Of the eight fosfomycin combinations studied, fosfomy-
cin combined with linezolid, minocycline, vancomycin or teicoplanin at their respective SBCs, exhibited
enhanced antibacterial activities (P,0.0001) when compared with the control group, and outperformed rifam-
picincombinations(P,0.01).ThekillingeffectsoffosfomycincombinationsattheirrespectiveRBCswerebetter
than those at their respective SBCs (P,0.05). Significantly enhanced killing effects were observed with fosfo-
mycin in combination with vancomycin or teicoplanin, compared with vancomycin or teicoplanin alone. For
10 randomly selected MRSA isolates, the results of colony counting in biofilms were comparable with those
of the MTT-staining method.
Conclusions: Fosfomycin enhanced the activities of linezolid, minocycline, vancomycin and teicoplanin. These
combinatorialtreatmentswereevenbetterthanrifampicincombinationregimens,andmayprovidetherapeutic
advantages in catheter-related or prosthetic joint infections.看了摘要，似乎跟mccA无关呢，是否楼主贴错了附件？
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