Two Novel Class I Integron Arrays in Pseudomonas aeruginosa

细菌耐药

This is an interesting story focus on novel cassette arrays of class 1 integrons in Pseudomonas aeruginosa.

Antimicrob Agents Chemother. 2012 Jan 30. [Epub ahead of print]
Two Novel Class I Integron Arrays Encoding IMP-18 Metallo-Beta-Lactamase Gene in Pseudomonas aeruginosa Clinical Isolates from Puerto Rico.
Martínez T, Vazquez GJ, Aquino EE, Goering RV, Robledo IE.
SourceDepartment of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, San Juan, PR, USA.

Abstract
During a beta-lactam resistance surveillance study, 12 IMP-18 positive Pseudomonas aeruginosa belonging to 9 different PFGE groups were identified. In nine isolates, a class I integron with a novel gene array was identified containing bla(IMP-18) and bla(OXA-224) while in two isolates the class I integron contained the bla(IMP-18) and bla(OXA-2) but in a new arrangement. Our findings show the dissemination of two novel class 1 integrons in P. aeruginosa from different regions of Puerto Rico.

蓝鱼o_0

PCR-based genotyping and sequecing analysis was widly used in molecular epidemiology, which should be paid much attention to.

liuaifen

身无半文，心忧天下；手释万卷，神交古人！

蓝鱼o_0

Integron是您以前研究的课题啊。您可以做个专题介绍，对于未知integron如何检测？

如何有效的利用网上资源，进行数据比对？

细菌耐药

蓝鱼o_0 发表于 2012-2-24 09:24

                               
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Integron是您以前研究的课题啊。您可以做个专题介绍，对于未知integron如何检测？

如何有效的利用网上资 ...

未知integron的检测上难度不大，测序是金标准:)

蓝鱼o_0

细菌耐药 发表于 2012-2-24 10:19

                               
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未知integron的检测上难度不大，测序是金标准:)

有没有比较好的筛选方法。直接全测序比较昂贵。
SSCP又比较麻烦啊。

细菌耐药

蓝鱼o_0 发表于 2012-2-24 10:53

                               
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有没有比较好的筛选方法。直接全测序比较昂贵。
SSCP又比较麻烦啊。

没有啊，其他方法就没有办法测未知的整合子啦，最多也就是对已知类型的整合子动些脑筋啊。