cfr gene 介导对利奈唑胺耐药的金黄色葡萄球菌

yzyy

Resistance to Linezolid Is Mediated by the cfr Gene in the First Report of an Outbreak of Linezolid-Resistant Staphylococcus aureus

Clinical Infectious Diseases Volume50, Issue6 Pp. 821-825.

Background. From April through June 2008, we identified 12 patients in the intensive care unit and 3 patients on other wards infected with methicillin-resistant Staphylococcus aureus that was also resistant to linezolid. We investigated the mechanism of resistance —point mutations in domain V of 23S ribosomal RNA (rRNA) or presence of the cfr gene —involved in the outbreak.

Methods. Strains for the study were obtained in the intensive care unit and other wards. Minimal inhibitory concentrations were determined using automated methods, the E-test, or dilution in Mueller-Hinton agar in accordance with Clinical and Laboratory Standards Institute guidelines. Strains were genotyped using pulsed-field gel electrophoresis and were sequenced to determine the presence of point mutations in 23S rRNA. The presence of the cfr gene was determined by specific polymerase chain reaction.

Results. The minimal inhibitory concentrations of linezolid ranged from 16 mg/L to 32 mg/L, and all the strains were susceptible to tigecycline, vancomycin, and daptomycin. Typing of strains sequentially isolated by pulsed-field gel electrophoresis showed that each patient carried only 1 clonal type of linezolid-resistant, methicillin-resistant S. aureus as detected by sequential isolations. The presence of the cfr gene was confirmed in all the isolates. Furthermore, sequencing of domain V of 23S rRNA showed that the most common mechanism of linezolid resistance reported to date, mutation G2576T, was not detected in any of the strains analyzed.

Conclusions. We report the presence of the cfr gene underlying the resistance mechanism involved in a clinical outbreak of linezolid-resistant S. aureus.



全文地址：
http://cid.oxfordjournals.org/content/50/6/821.long

clarck7

这下好玩了。利奈坐胺也难以支持太久时间呀。昨天看资料时，发现亚胺培南是1984年投入米国医疗市场的，短短的时间里就出现了耐imp的，利奈坐胺在我国还能坚持多久呢？？