高通量全基因组测序分析鲍曼不动杆菌感染暴发的流行情况

David

请大家关注用新一代测序技术进行细菌同源性分析的最新进展。目前成本较高，但未来有可能代替MLST和PFGE作为分子分型的新“金标准”。
J Hosp Infect. 2010 May;75(1):37-41. Epub 2010 Mar 17.

High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak.
Lewis T, Loman NJ, Bingle L, Jumaa P, Weinstock GM, Mortiboy D, Pallen MJ.

Department of Medical Microbiology, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.

Abstract
Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting. Copyright (c) 2010. Published by Elsevier Ltd.

PMID: 20299126 [PubMed - in process]