Langmuir：新型抗菌涂层可显著降低手术缝合处感染率

婉若秋水

来源：丁香园 作者：猎人vs狼

外科手术缝合处急需抗菌效果更好的涂层，有科学家称已发现一种抗菌效果比当前被广泛应用的抗菌涂层强大1000倍的新型涂层。ACS旗下杂志Langmuir刊发了此报道。

来自麻省大学安赫斯特分校的Gregory Tew教授与其同事解释道，外科手术切口部位感染是导致患者术后住院时间增加和住院费用上升最常见的并发症。含三氯生的抗菌涂层被广泛使用，但是由于长期的应用已经出现可耐受其影响的细菌株。三氯生可被人体吸收，这也增加了（使用者）对其不良健康影响的担忧。三氯生的另一个缺点：它虽然可以减慢细菌的生长，但是不能杀死已经存在的细菌。这也是为什么科学家要转而研究出PAMBM----源于一种天然存在的具有广谱杀菌作用的抗微生物肽。由于这种新物质的作用方式（不同），PAMBM导致细菌耐药从而出现所谓的超级细菌的几率非常低。

根据报道阐述的实验室检测结果，相比于三氯生，PAMBM能更大程度的降低细菌数量。在与三氯生涂层的短兵相接中，PAMBM涂层被证明具有更好的抗菌效果。研究人员称：“记录表明细菌对当前药物的耐药性持续上升，并对三氯生产生抵抗力，所以急需发现一种新的具有生物医疗设备涂层活性的抗微生物药物。”

西安猫

谢谢宛若秋水老师发的最新研究成果。找到了英文的摘要。

Langmuir. 2012 Aug 21;28(33):12134-9. Epub 2012 Aug 9.
New bactericidal surgical suture coating.
Li Y, Kumar KN, Dabkowski JM, Corrigan M, Scott RW, Nüsslein K, Tew GN.
Source

Department of Polymer Science & Engineering, ‡Department of Microbiology, ∥Department of Veterinary &Animal Science, and ⊥Molecular & Cellular Biology Program, University of Massachusetts , Amherst, Amherst, Massachusetts 01003, United States.
Abstract

This paper demonstrates the effectiveness of a new antimicrobial suture coating. An amphiphilic polymer, poly[(aminoethyl methacrylate)-co-(butyl methacrylate)] (PAMBM), inspired by antimicrobial peptides, was bactericidal against S. aureus in time-kill experiments. PAMBM was then evaluated in a variety of polymer blends using the Japanese Industrial Standard (JIS) method and showed excellent antimicrobial activity at a low concentration (0.5 wt %). Using a similar antimicrobial coating formula to commercial Vicryl Plus sutures, disk samples of the coating material containing PAMBM effectively killed bacteria (98% reduction at 0.75 wt %). Triclosan, the active ingredient in Vicryl Plus coatings, did not kill the bacteria. Further Kirby-Bauer assays of these disk samples showed an increasing zone of inhibition with increasing concentration of PAMBM. Finally, the PAMBM-containing coating was applied to sutures, and the morphology of the coating surface was characterized by SEM, along with Vicryl and uncoated sutures. The PAMBM-containing sutures killed bacteria more effectively (3 log(10) reduction at 2.4 wt %) than Vicryl Plus sutures (0.5 log(10) reduction).