台湾EV71-IgM资料（翻译有奖）Responses of IgM for Enterovirus 71 Infection

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rapid serological assay was developed for detection of specific IgM to enterovirus 71, a human picornavirus that is usually associated  with severe central nervous system complications. The sensitivity and specificity of this ‘‘inhouse’’ m-capture enzyme linked-immunosorbentassay was assessed by testing 213 serum samples. With the conventional virus culture as a standard method, the sensitivity and specificity were 91.5 and 93.1%, respectively, for this newly developed immunoassay. This methodallows for detection of the IgM responses from the patients either infected by genotype B or genotype C of enterovirus 71. IgM can be detected as early as  the second day from the onset of disease. IgM responses exhibit100%positive rate from enterovirus  71-infected patients with complications, including encephalitis, meningitis, polio-like syndrome, pulmonary edema, and fatal cases. These  findings suggest that detection of specific IgM by  the use of enzyme linked-immunosorbent assay is a rapid and valuable way for the diagnosis of enterovirus 71 infection.
J. Med. Virol. 68:574– 580, 2002.
2002 Wiley-Liss, Inc.
感谢David版主去年年底帮助查到此篇文献。

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长庚大学医疗技术学院，桃园，台湾

长庚医院临床病毒学实验室，临床病理学，桃园，台湾

长庚儿童医院儿童传染病科，儿科，桃园，台湾部

开发了一种快速的EV71特异性IgM血清学检测方法，EV71是一种通常引起严重CNS并发症人类小RNS病毒。通过213份血清样本评估捕获ELISA的敏感性和特异性。与常规病毒培养标准方法相比，这种新的免疫测定的敏感性和特异性分别为91.5%和93.1％。这种方法可以检测基因型B型或C型EV71感染病人的IgM 应答。IgM可以早在发病第二天检测出来。 EV71感染并发症，包括脑炎、脑膜炎、脊髓灰质炎样综合征、肺水肿和死亡患者IgM阳性率100％。这些发现表明了使用ELISA检测特异性IgM对EV71感染的诊断是快速、有价值的方法。

   我的英语差不多还给老师了， 论坛藏龙卧虎，请高手翻译,有奖！