文摘大家译：NICU产超广谱β -内酰胺酶粘质沙雷氏菌和肺炎克雷伯菌感染的危险因素

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Risk factors for extended-spectrum β-lactamase-producing Serratia marcescens and Klebsiella pneumoniae acquisition in a neonatal intensive care unit Journal of Hospital Infection
Volume 67, Issue 2, October 2007, Pages 135-141

V. Crivaroa, M. Bagattinia, M.F. Salzaa, F. Raimondib, F. Rossanoc, M. Triassia and R. Zarrillia, d, ,  
aDepartment of Preventive Medical Sciences, Hygiene Section, University ‘Federico II’, Naples, Italy
bDepartment of Paediatrics, University ‘Federico II’, Naples, Italy
cDepartment of Molecular and Cellular Biology and Pathology, University ‘Federico II’, Naples, Italy
dCEINGE Advanced Biotechnologies, Naples, Italy
Received 31 May 2007;  accepted 26 July 2007.  Available online 19 September 2007.

Summary
We investigated the molecular epidemiology of gentamicin-resistant, extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae and Serratia marcescens, and risk factors associated with their acquisition in a neonatal intensive care unit (NICU) of a university hospital in Italy. During the study period (April–November 2004), S. marcescens was responsible for six infections and 31 colonisations, while K. pneumoniae was responsible for six infections and 103 colonisations. Concurrent isolation of both organisms occurred in 24 neonates. Molecular typing identified one major pulsed-field gel electrophoresis pattern each for S. marcescens and K. pneumoniae strains isolated during the study period. An 80 kb plasmid containing blaSHV-12, blaTEM-1 and aac(6′)-Ib genes, isolated from both S. marcescens and K. pneumoniae strains, and showing identical restriction profiles, transferred resistance to third-generation cephalosporins to a previously susceptible Escherichia coli host. Birthweight, gestational age and use of invasive devices were significantly associated with S. marcescens and K. pneumoniae acquisition on univariate analysis, while empiric antimicrobial treatment with ampicillin and gentamicin, and duration of hospital stay, proved to be the only independent risk factors. In conclusion, conjugal plasmid transfer and empiric antimicrobial therapy with ampicillin and gentamicin might have contributed to the selection and spread of gentamicin-resistant ESBL-producing Enterobacteriaceae in the NICU.

Keywords: Nosocomial infections; Antimicrobial resistances; Genotyping; Horizontal gene transfer; Risk factor analysis

青暮

着翻译了下，有些地方感觉不通顺，希望版主指点～～
Summary
摘要：
We investigated the molecular epidemiology of gentamicin-resistant, extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae and Serratia marcescens, and risk factors associated with their acquisition in a neonatal intensive care unit (NICU) of a university hospital in Italy. 我们对意大利一所大学附属医院新生儿重症监护病房（NICU）内耐庆大霉素、产超广谱β -内酰胺酶粘质沙雷氏菌和肺炎克雷伯菌的分子流行病学及其危险因素进行研究。During the study period (April–November 2004), S. marcescens was responsible for six infections and 31 colonisations, while K. pneumoniae was responsible for six infections and 103 colonisations. 在研究期间（2004年4月至11月），发现有6例感染和31例定植由粘质沙雷菌引起，而肺炎克雷伯菌引起了6例感染和103例定植。Concurrent isolation of both organisms occurred in 24 neonates.其中在24个新生儿中同时分离出这两种菌。 Molecular typing identified one major pulsed-field gel electrophoresis pattern each for S. marcescens and K. pneumoniae strains isolated during the study period.在研究期间对每一个粘质沙雷菌和肺炎克雷伯菌分离株经脉冲场凝胶电泳后的一段主要电泳图谱进行分型鉴定。An 80 kb plasmid containing blaSHV-12, blaTEM-1 and aac(6′)-Ib genes, isolated from both S. marcescens and K. pneumoniae strains, and showing identical restriction profiles, transferred resistance to third-generation cephalosporins to a previously susceptible Escherichia coli host. 从粘质沙雷菌和肺炎克雷伯菌都分离出一段80kb长度的质粒，其中包含blaSHV-12, blaTEM-1 and aac(6′)-Ib 基因，显示出相同的限制概况，该质粒使先前敏感的大肠埃希菌宿主转移为对第三代头孢菌素耐药。Birthweight, gestational age and use of invasive devices were significantly associated with S. marcescens and K. pneumoniae acquisition on univariate analysis, while empiric antimicrobial treatment with ampicillin and gentamicin, and duration of hospital stay, proved to be the only independent risk factors. 在单变量分析中，出生体重、胎龄和侵入设备的使用与粘质沙雷菌和肺炎克雷伯菌的产生明显相关。而氨苄西林和庆大霉素的经验抗菌治疗、住院时间，被证明是唯一的独立危险因素。In conclusion, conjugal plasmid transfer and empiric antimicrobial therapy with ampicillin and gentamicin might have contributed to the selection and spread of gentamicin-resistant ESBL-producing Enterobacteriaceae in the NICU.
结论认为，接合质粒转移、氨苄西林和庆大霉素的经验抗菌治疗可能促使耐庆大霉素、产超广谱β -内酰胺酶肠杆菌科在新生儿重症监护病房（NICU）内产生和传播。
Keywords: Nosocomial infections; Antimicrobial resistances; Genotyping; Horizontal gene transfer; Risk factor analysis
关键词：医院感染；抗生素耐药性；基因分型；水平基因转移；危险因素分析

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迎继续参与其他文摘翻译！

右手心

迎您经常来参加:loveliness:

青暮

呵，一定一定。

David

he translation is just OK.:)  And warmly welcome you to join us. But in this sentence" Molecular typing identified one major pulsed-field gel electrophoresis pattern each for S. marcescens and K. pneumoniae strains isolated during the study period." I think it should be more comfortable if translated as this:"研究中我们用分子分型的方法对分离出的粘质沙雷菌和肺炎克雷伯菌进行分析,均确认含有同一条主要的脉冲场凝胶电泳条带。"
BTW, "showing identical restriction profiles" means"显示有相同的限制性内切酶酶谱。":handshake

青暮

常感谢David的解释。还是你翻译的通顺～～