几种血培养系统比较
size=3]Table 3. Blood culture systems_______________________________________________________________________________________________________________________
Method Aerobes Anaerobes Yeast Fungi Mycobacteria Comments
_______________________________________________________________________________________________________________________________________________________________________
Conventional broth-in-bottle ++ ++ +/++ + + Slower than automated systems
Broth-in-bottle with +++ ++/+++ ++/+++ + +a,+++b Speeds time to detection campared
continuous monitoring with intermittent monitoring
Lysis-centrifugation(Isolatorc)c ++/+++ + +++ +++ +++ Volume cultured is 10 mL
Antibiotic removal systems ++ +/++ ++ + + Greatest yield on staphylococci and yeast
(resin bottles) compared with conventional systems
________________________________________________________________________________________________________________________-
+, not recommended; ++, acceptable; +++, best available method.
a,Using standard blood culture bottles; b,using special mycobacterial bottles; c,Isolator (Inverness Medical Innovations, Waltham, MA).
上面是2008年4月“the American College of Critical CareMedicine”和 “the Infectious Diseases Society of America（IDSA）"联合发布的对重症病人新出现的发热评价指南中提到的几种血培养系统，第二种和第三种不知道原理具体是怎样的？望赐教！
附全文：
attach]5280
第一种是传统肉汤基础的血培养瓶:有单纯液体和固液双相培养基两种形式,其中双相培养基明显优于单纯液体的培养基,可直接取固相上的菌落进行药敏,赢得时间至少24小时.
第二种是肉汤基础培养瓶辅助连续监视器:是采用单纯液体培养基辅助瓶外连续自动监视检测系统,是现在普遍采用的形式.缺点是在报阳性后必需传代至固体培养基上再培养24小时以上,又延长了培养时间.
第三种是溶解离心系列:在一灭菌离心管中含溶血卵磷脂,皂素,长链脂肪酸,缓冲剂,抗生素灭活剂等物质.将10ml血液注入试管中,混匀孵育让血细胞完全溶解后离心,弃上清液,取沉渣直接接种固体培养基或转入液体增菌培养基中增菌后在传至固体培养基上.对真菌和在液体培养基中生长不良的细菌(如流感杆菌,脑膜炎球菌,分支杆菌等),可明显提高阳性率,缩短培养时间.缺点是采血量大,由于中间多了一个步骤,污染的机会就增加了.
第四种是抗生素消除系统(即复合树脂培养瓶):瓶中含有多种混合树脂,主要作用是对以离子态存在的抗生素药物具有很强的吸附力,能大大降低血液中残留抗生素对细菌生长的不利影响.从而提高阳性率.适合已经使用过抗生素的病例.缺点是价格昂贵.
页:
[1]