有谁知道capillary吗?
有谁知道capillary吗?是一种鉴别结核和非结核分枝杆菌的方法。有谁知道或用过,介绍一下它的原理和具体方法好吗? 找到答案了,可是看不懂,哪位能帮我翻译一下?[ 本帖最后由 莲雾 于 2007-8-15 13:33 编辑 ]
回复 #2 莲雾 的帖子
目前只知道上面介绍的特长,大意如下:这个方法灵敏度和特异性很好。不需要复杂精密的仪器,操作简单。短时间内(15分钟)就可见结果。该方法试剂使用方便并可避免试剂浪费。
操作方法就和一般的胶体金方法相似,看看图就知道了。
其余的部分我会继续努力的~解释的不是很好,望版主见谅!:P capillia是鉴定结核分支杆菌的新技术,以下是cappillia的资料。
台大医院介绍Capilia TB的2007年新文献
4: Diagn Microbiol Infect Dis. 2007 Dec;59(4):395-9. Epub 2007 Sep 18.Related Articles, Links
Performance assessment of the Capilia TB assay and the BD ProbeTec ET system for rapid culture confirmation of Mycobacterium tuberculosis.
Wang JY, Lee LN, Lai HC, Hsu HL, Jan IS, Yu CJ, Hsueh PR, Yang PC.
Internal Medicine, National
Taiwan
University
Hospital, Taipei 100, Taiwan.
Because of the increasing numbers of nontuberculous mycobacterial isolates from clinical specimens, rapid and accurate methods for culture confirmation of Mycobacterium tuberculosis are urgently needed. The study evaluated the performance of the Capilia TB immunochromatographic assay (TAUNS, Numazu, Japan) for culture confirmation of M. tuberculosis using 242 culture-positive liquid media in 2 mycobacterial laboratories from November 2005 to February 2006. Among the 242 samples, 183 were also tested with the BD ProbeTec ET (CTB) assay (Becton Dickinson, Sparks, MD). The results of both assays were compared to the culture results and to each other. The overall sensitivity and specificity of the Capilia TB assay were 98.6% and 97.9%, respectively, and for the CTB assay were 97.3% and 97.1%, respectively. The positive and negative predictive values for the Capilia TB assay were 98.6% and 97.9%, respectively, and for the CTB assay were 98.2% and 95.8%, respectively. Among the 183 samples tested with both assays, 8 had discrepant results, including Capilia-TB-false-positive in 2, CTB-false-positive in another 2, CTB-false-negative in 2, Capilia TB-false-negative in 1, and both assays with false-negative results in the remaining one. This study demonstrated that the Capilia TB assay has a similar diagnostic value with the CTB assay. In addition, with the immunochromatographic method, it is less time-consuming and does not require other laboratory equipment.
Capilia與ProbeTec鑑定結核分枝菌比較
本文主要是利用Capilia免疫膠體金方法與ProbeTec的分子生物SDA方法,比較鑑定結核分枝菌的正確性.結果顯示:兩種方法的敏感性與特異性幾乎相同.建議可以使用CAPILIA的方法於培養陽性後十五分鐘快速鑑定MTB, 而且不需要有特別的設備. 终于用了传说中的Capilia,用后感觉非常简便、快速,准确性颇高(只是感觉,还没有足够数据统计)。技术方法是免疫层析胶体金方法。发个图片....:lol 能否翻译一下我们也想学习.回复 #8 攀峰 的帖子
6楼已将基本内容翻了一遍:)
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