Microbiology scientist introduction 3: DAOGUO ZHOU

细菌耐药

Professor
LILY 1-235
Phone: 765-494-8159

Pathogenic Salmonella strains cause food poisoning, gastrointestinal inflammation, typhoid fever, and septicemia in humans. The bacteria achieve this by making a panel of specialized proteins that allows it to invade non-phagocytic intestinal epithelial cells, the first step in the disease process. Previous research has established that upon contact with the intestinal epithelial cells, Salmonella injects a set of proteins into the host cells through the bacterially encoded type III protein secretion system. These proteins subvert host cell signal transduction pathways to induce profuse actin cytoskeletal rearrangements and membrane ruffling, leading to the uptake of the bacterium. This uptake process is a highly regulated event and requires the coordinated action of the injected bacterial proteins as well as host proteins. My laboratory is interested in identifying and characterizing bacterial and host cellular proteins that are involved in Salmonella -induced actin cytoskeletal rearrangements, especially cellular proteins whose activities are modulated by Salmonella proteins. Studying the interaction between bacteria and their hosts has emerged as one of the most exciting areas of cellular microbiology. It will not only broaden our understanding of bacterial pathogenesis, but also will provide unexpected insights into basic host cellular functions, such as the cytoskeletal rearrangements and signal transduction pathways controlling cell movement.

Education
Ph.D., University of Iowa, 1995
Other Activities
Faculty Presentations
Eighth Annual Midwest Microbial Pathogenesis Meeting, University of Illinois, Urbana-Champaign, October, 2001.
Department of Molecular Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio, February, 2001.
Department of Pathology, Gastrointestinal Pathology, Wayne State University School of Medicine, Detroit, Michigan, March, 2001.
Indiana University-Purdue University, Indianapolis, Indiana, November, 2001.
The International Symposium on Frontiers of Life Sciences and Biotechnology, Wuhan University, Wuhan, China, September, 2003.
Fudan University, Shanghai, China, October, 2003.
Eastern Illinois University, Charleston, Illinois, October, 2003.
The University of Kentucky, Lexington, KY, November, 2003.
Fudan Medical College, Shanghai, China, November, 2003.
Indiana University at Bloomington, Bloomington, Indiana, January, 2004.
Medical College of Wisconsin, Milwaukee, Wisconsin, March, 2004.
Department of Biological Sciences, Purdue University, October, 2004.

细菌耐药

Journal
Diao, J., Zhang, Y., Huibregtse, J. M., Zhou, D., Chen, J. 2008. Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Nat Struct Mol Biol. 15: 65-70.
Chang, J., Myeni, S. K., Lin, T. L., Wu, C. C., Staiger, C. J., Zhou, D. 2007. SipC multimerization promotes actin nucleation and contributes to Salmonella-induced inflammation. Mol Microbiol. 66: 1548-1556.
Dai, S., Zhang, Y., Weimbs, T., Yaffe, M. B., Zhou, D. 2007. Bacteria-generated PtdIns(3)P Recruits VAMP8 to Facilitate Phagocytosis. Traffic. 8: 1365-1374.
Higashide, W., Zhou, D. 2006. The first 45 amino acids of SopA are necessary for InvB binding and SPI-1 secretion. J Bacteriol. 188: 2411-2420.
Hu, H., Sa, Q., Koehler, T. M., Aronson, A. I., Zhou, D. 2006. Inactivation of Bacillus anthracis spores in murine primary macrophages. Cell Microbiol. 8: 1634-1642.
Zhang, Y., Higashide, W. M., McCormick, B. A., Chen, J., Zhou, D. 2006. The inflammation-associated Salmonella SopA is a HECT-like E3 ubiquitin ligase. Mol Microbiol. 62: 786-793.
Zuo, X., Zhang, J., Zhang, Y., Hsu, S. C., Zhou, D., Guo, W. 2006. Exo70 interacts with the Arp2/3 complex and regulates cell migration. Nat Cell Biol. 8: 1383-1388.
Chang, J., Chen, J., Zhou, D. 2005. Delineation and characterization of the actin nucleation and effector translocation activities of Salmonella SipC. Mol Microbiol. 55: 1379-1389.
Zhang, Y., Higashide, W., Dai, S., Sherman, D. M., Zhou, D. 2005. Recognition and ubiquitination of Salmonella type III effector SopA by a ubiquitin E3 ligase, HsRMA1. J Biol Chem. 280: 38682-38688.
Dai, S., Sarmiere, P. D., Wiggan, O., Bamburg, J. R., Zhou, D. 2004. Efficient Salmonella entry requires activity cycles of host ADF and cofilin. Cell Microbiol. 6: 459-471.
Dai, S., Zhou, D. 2004. Secretion and function of Salmonella SPI-2 effector SseF require its chaperone, SscB. J Bacteriol. 186: 5078-5086.
Galkin, V. E., Orlova, A., VanLoock, M. S., Zhou, D., Galan, J. E., Egelman, E. H. 2002. The bacterial protein SipA polymerizes G-actin and mimics muscle nebulin. Nat Struct Biol. 9: 518-521.
Higashide, W., Dai, S., Hombs, V. P., Zhou, D. 2002. Involvement of SipA in modulating actin dynamics during Salmonella invasion into cultured epithelial cells. Cell Microbiol. 4: 357-365.
Zhou, D. 2001. Collective efforts to modulate the host actin cytoskeleton by Salmonella type III-secreted effector proteins. Trends Microbiol. 9: 567-569 discussion 569-570.
Zhou, D., Chen, L. M., Hernandez, L., Shears, S. B., Galan, J. E. 2001. A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization. Mol Microbiol. 39: 248-259.
Zhou, D., Galan, J. 2001. Salmonella entry into host cells: the work in concert of type III secreted effector proteins. Microbes Infect. 3: 1293-1298.
GalÃin, J. E., Zhou, D. 2000. Striking a balance: modulation of the actin cytoskeleton by Salmonella. Proc Natl Acad Sci U S A. 97: 8754-8761.
Mitra, K., Zhou, D., Galan, J. E. 2000. Biophysical characterization of SipA, an actin-binding protein from Salmonella enterica. FEBS Lett. 482: 81-84.
Zhou, D., Hardt, W. D., Galan, J. E. 1999. Salmonella typhimurium encodes a putative iron transport system within the centisome 63 pathogenicity island. Infect Immun. 67: 1974-1981.
Zhou, D., Mooseker, M. S., Galan, J. E. 1999. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proc Natl Acad Sci U S A. 96: 10176-10181.
Zhou, D., Mooseker, M. S., Galan, J. E. 1999. Role of the S. typhimurium actin-binding protein SipA in bacterial internalization. Science. 283: 2092-2095.
Estabrook, M. M., Zhou, D., Apicella, M. A. 1998. Nonopsonic phagocytosis of group C Neisseria meningitidis by human neutrophils. Infect Immun. 66: 1028-1036.
Apicella, M. A., Ketterer, M., Lee, F. K., Zhou, D., Rice, P. A., Blake, M. S. 1996. The pathogenesis of gonococcal urethritis in men: confocal and immunoelectron microscopic analysis of urethral exudates from men infected with Neisseria gonorrhoeae. J Infect Dis. 173: 636-646.
Zhou, D., Apicella, M. A. 1996. Plasmids with erythromycin resistance and catechol 2,3-dioxygenase- or beta-galactosidase-encoding gene cassettes for use in Neisseria spp. Gene. 171: 133-134.
Lee, F. K., Stephens, D. S., Gibson, B. W., Engstrom, J. J., Zhou, D., Apicella, M. A. 1995. Microheterogeneity of Neisseria lipooligosaccharide: analysis of a UDP-glucose 4-epimerase mutant of Neisseria meningitidis NMB. Infect Immun. 63: 2508-2515.
Stephens, D. S., McAllister, C. F., Zhou, D., Lee, F. K., Apicella, M. A. 1994. Tn916-generated, lipooligosaccharide mutants of Neisseria meningitidis and Neisseria gonorrhoeae. Infect Immun. 62: 2947-2952.
Zhou, D., Lee, N. G., Apicella, M. A. 1994. Lipooligosaccharide biosynthesis in Neisseria gonorrhoeae: cloning, identification and characterization of the alpha 1,5 heptosyltransferase I gene (rfaC). Mol Microbiol. 14: 609-618.
Zhou, D., Stephens, D. S., Gibson, B. W., Engstrom, J. J., McAllister, C. F., Lee, F. K., Apicella, M. A. 1994. Lipooligosaccharide biosynthesis in pathogenic Neisseria. Cloning, identification, and characterization of the phosphoglucomutase gene. J Biol Chem. 269: 11162-11169.
Kim, J. J., Zhou, D., Mandrell, R. E., Griffiss, J. M. 1992. Effect of exogenous sialylation of the lipooligosaccharide of Neisseria gonorrhoeae on opsonophagocytosis. Infect Immun. 60: 4439-4442.

jerkran

可否翻译下，老师

沈艳梨

老师，帮翻译一下，谢谢。

snxmm77

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