社区和实验室内，流感病毒在手上和污物中的存活
Survival of influenza virus on hands and fomites in community and laboratory settingsDhritiman V. Mukherjee, MS, PhD
AFFILIATIONS
Columbia University School of Nursing, New York, NY
Mailman School of Public Health, Columbia University, New York, NY
Address correspondence to Dhritiman V. Mukherjee, MS, PhD, Columbia University School of Nursing, 630 West 168th Street, New York, NY 10032.
, Bevin Cohen, MPH, Mary Ellen Bovino, BSN, Shailesh Desai, MS, Susan Whittier, PhD, Elaine L. Larson, RN, PhD
published online 24 January 2012.
Background
Transmission dynamics modeling provides a practical method for virtual evaluation of the impact of public health interventions in response to prospective influenza pandemics and also may help determine the relative contribution of different modes of transmission to overall infection rates. Accurate estimates of longevity for all forms of viral particles are needed for such models to be useful.
Methods
We conducted a time course study to determine the viability and longevity of H1N1 virus on naturally contaminated hands and household surfaces of 20 individuals with laboratory-confirmed infection. Participants coughed or sneezed into their hands, which were sampled immediately and again after 5, 10, and 30 minutes. Samples also were obtained from household surfaces handled by the participants immediately after coughing/sneezing. Clinically obtained H1N1 isolates were used to assess the viability and longevity of the virus on various artificially inoculated common household surfaces and human hands in a controlled laboratory setting. Viral detection was achieved by culture and real-time reverse-transcriptase polymerase chain reaction.
Results
The results suggest that H1N1 does not survive long on naturally contaminated skin and fomites, and that secretions deposited on hands by coughing or sneezing have a concentration of <2.15 × 10 to 2.94 × 10 TCID50/mL.
Conclusions
These data can be used to estimate the relative contribution of direct and indirect contact transmission on overall infection rates.
Key Words: H1N1, Transmission, Detection, Viability
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